New prognostic markers of preeclampsia and anemia in obese pregnant women

• Agamurad A. Orazmuradov
• Sergey G. Morozov
• Irina V. Bekbaeva
• Nozimabonu M. Zokirova
• Khalid Haddad
• Sergey I. Kyrtikov
• Ekaterina V. Mukovnikova

Abstract

The aim of the study was to identify new prognostic markers of preeclampsia and anemia in obese women.

Material and methods. A prospective cohort study was conducted, which included 159 pregnant women at full–term gestation (37.0–41.0 weeks). The 1^st group included pregnant women with obesity and preeclampsia (n=40), the 2^nd – patients with anemia and obesity (n=39), the 3^rd – obese women (n=42), the 4^th – normal-weight pregnant women without a burdened somatic history (n=38). For protein quantification by mass spectrometry, a Normalised Spectral Adundance Factor (NSAF) was calculated for each identified protein.

Results. Among the identified proteomic spectrum, 8 proteins were identified, the differences in the spectral prevalence of which significantly differed from the control group. For pregnant women with obesity and PE, significant coefficients of change were identified for 4 protein molecules when comparing their spectral prevalence with those in obese patients: Immunoglobulin kappa variable 2D-28, Zinc-alpha-2-glycoprotein, Lumican, Apolipoprotein C-IV. However, the differences were statistically significant only for Zinc-alpha-2-glycoprotein. Keratin type II cytoskeletal 1 protein was significantly significant for a cohort of pregnant women with anemia and obesity.

Conclusion. The most promising serological marker for the prognosis of preeclampsia in obesity is Zincalpha-2-glycoprotein, for the prognosis of anemia in obesity – Keratin type II cytoskeletal 1. The results obtained demonstrate the need for additional studies to investigate the proteome of obese pregnant women.

Keywords:obesity; anemia; preeclampsia; mass spectrometry; proteomic analysis

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