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4 . 2022

Taxonomic profile of the endometrial microbiome in chronic endometritis

Abstract

The aim of the study to determine the taxonomic profile of the endometrial microbiome in chronic endometritis and identify the most significant combinations of various infections.

Design: observational, cohort, closed research.

Material and methods. 88 patients of reproductive age with infertility were examined: group I (n=56) – the main one, patients with infertility on the background of chronic endometritis, group II (n=32) – control, patients without chronic endometritis. The examination included anamnestic data, patient complaints, general anamnesis and gynecological status, a comprehensive microbiological study of the microbiome and virome of the endometrium and the vaginal discharge.

Results. There was no significant difference in species composition in the endometrial microbiome between the examined groups, and the degree of contamination in most patients did not exceed 104 GE/ml. Herpesviruses were represented by HSV and CMV and were significantly more common in group I – in 19.64% of patients than in group II – in 9.38% of patients. Comparative characteristics of the species composition of the microbiome of the endometrium and vagina showed that in endometrial biopsies the degree of contamination was lower and for the most part did not exceed 104, whereas in the vagina the titer was 107–108, while the species composition had practically no differences. However, in some patients, endometrial biopsies revealed Ureaplasma urealyticum, Mycoplasma hominis, which were absent in the vagina.

Conclusion. The microbiome of the endometrium is represented by conditionally pathogenic flora: Enterobacteriaceae spp., Streptococcus spp., Gardnerella vaginalis, Ureaplasma spp. with a low degree of contamination, while in patients with chronic endometritis Lactobacillus spp. in the uterine cavity, they were detected 3 times less often.

Keywords:endometrial microbiome; herpesviruses; chronic endometritis; infertility

Funding. The study had no sponsor support.

Conflict of interest. The authors declare no conflict of interest.

Contribution. Concept development (idea formation, formulation and development of key goals and objectives; conducting a study), analysis and interpretation of the data obtained – Karakhalis L.Yu., Kononenko T.S.; preparation and editing of the text – a critical review of the manuscript with the introduction of a valuable comment of intellectual content – Karakhalis L.Yu.; preparation and editing of the text (drafting of the manuscript; participation in scientific design) – Kononenko T.S.; development of methodology – Karakhalis L.Yu., Kononenko T.S.; approval of the final version of the article, taking responsibility for all aspects of the work, the integrity of all parts of the article and its final version – Karakhalis L.Yu., Kononenko T.S.

For citation: Karakhalis L.Yu., Kononenko T.S. Taxonomic profile of the endometrial microbiome in chronic endometritis. Akusherstvo i ginekologiya: novosti, mneniya, obuchenie [Obstetrics and Gynecology: News, Opinions, Training]. 2022; 10 (4): 23–30. DOI: https://doi.org/10.33029/2303-9698-2022-10-4-23-30 (in Russian)

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CHIEF EDITORS
CHIEF EDITOR
Sukhikh Gennadii Tikhonovich
Academician of the Russian Academy of Medical Sciences, V.I. Kulakov Obstetrics, Gynecology and Perinatology National Medical Research Center of Ministry of Healthсаre of the Russian Federation, Moscow
CHIEF EDITOR
Kurtser Mark Arkadievich
Academician of the Russian Academy of Sciences, MD, Professor, Head of the Obstetrics and Gynecology Subdepartment of the Pediatric Department, N.I. Pirogov Russian National Scientific Research Medical University, Ministry of Health of the Russian Federation
CHIEF EDITOR
Radzinsky Viktor Evseevich
Corresponding Member of the Russian Academy of Sciences, MD, Professor, Head of the Subdepartment of Obstetrics and Gynecology with a Course of Perinatology of the Medical Department in the Russian People?s Friendship University

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