Characteristics of the microvesule composition in physiological pregnancy and pregnancy complicated by the intrauterine growth restriction

• Mikayelyan Asmik G.
• Marey Maria V.
• Sukhanova Yulia A.
• Vishnyakova Polina A.
• Bulatova Yulia S.
• Pyataeva Sofia V.
• Tsvirkun Dariya V.
• Nana K. Tetruashvili
• Mikhail Yu. Vysokikh

Abstract

Aim of the study: аnalysis of the composition of microvesicles at different periods of gestation during physiological pregnancy and pregnancy with intrauterine growth restriction.

Material and methods. Plasma microvesicle fraction of 19 pregnant women with intrauterine growth restriction and 10 women with physiological pregnancy was studied. The microvesicular fraction of plasma was obtained by the method of differential centrifugation of the blood of patients. The determination of the level of proteins of interest was carried out by the method of western blot analysis with detection of chemiluminescence.

Results. It was found that in microvesicles of patients with intrauterine growth restriction, there is a decrease in the content of important mitochondrial proteins responsible for maintaining adequate activity of mitochondria, preserving their integrity and biogenesis (VDAC1, TFAM, MFN2).

Conclusion. The obtained results allow to make a conclusion about a pronounced violation of mitochondrial biogenesis in pregnant women with intrauterine growth restriction, which probably makes a serious contribution to the development of pathology and its progression.

Keywords:intrauterine growth restriction, placental insufficiency, mitochondria, pregnancy, microvesicles

References

1. Sharma D., Shastri S., Sharma P. Intrauterine growth restriction: antenatal and postnatal aspects. Clin Med Insights Pediatr. 2016; 10: 67-83. doi: https://doi.org/10.4137/CMPed.S40070 .

2. Romo A., Carceller R., Tobajas J. Intrauterine growth retardation (IUGR): epidemiology and etiology. Pediatr Endocrinol Rev. 2009; 6, suppl. 3: 332-6. doi: http://www.ncbi.nlm.nih.gov/pubmed/19404231 .

3. Azhibekov S.A., Putilova N.V., Tretyakova T.B., Pestryaeva L.A. Role of the inherited characteristics of energy metabolism in the development of placental insufficiency with an outcome to intrauterine growth restriction. Akusherstvo i Ginekologiya [Obstetrics and Gynecology]. 2016; (11): 11-5. doi: http://dx.doi.org/10.18565/aig.2016.11.11-5 (in Russian)

4. La Rocca C., Carbone F., Longobardi S., Matarese G. The immunology of pregnancy: regulatory T cells control maternal immune tolerance toward the fetus. Immunol Lett. 2014; 162 (1): 41-8. doi: https://doi.org/10.1016/J.IMLET.2014.06.013 .

5. Stahl P.D., Raposo G. Exosomes and extracellular vesicles: the path forward. Essays Biochem. 2018; 62 (2): 119-24. doi: https://doi.org/10.1042/EBC20170088 .

6. Dimuccio V., Ranghino A., Barbato L.P., Fop F., et al. Urinary CD133+ extracellular vesicles are decreased in kidney transplanted patients with slow graft function and vascular damage. PLoS One. 2014; 9 (8). doi: https://doi.org/10.1371/journal.pone.0104490 .

7. Kornek M., Schuppan D. Microparticles: modulators and biomarkers of liver disease. J Hepatol. 2012; 57 (5): 1144-6. doi: https://doi.org/10.1016/j.jhep.2012.07.029 .

8. Rackov G., Garcia-Romero N., Esteban-Rubio S., Carrion-Navarro J., et al. Vesicle-mediated control of cell function: the role of extracellular matrix and microenvironment. Front Physiol. 2018; 9. doi: https://doi.org/10.3389/fphys.2018.00651 .

9. Valad H., Ekstrom K., Bossios A., Sjostrand M., et al. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat. Cell Biol. 2007; 9 (6): 654-9. doi: https://doi.org/10.1038/ncb1596 .

10. Garcia-Romero N., Esteban-Rubio S., Rackov G., Carrion-Navarro J., et al. Extracellular vesicles compartment in liquid biopsies: clinical application. Mol Aspects Med. 2018; 60: 27-37. doi: https://doi.org/10.1016/j.mam.2017.11.009 .

11. Thery C. Exosomes: secreted vesicles and intercellular communications. F1000 Biol Rep. 2011; 3. doi: https://doi.org/10.3410/b3-15 .

12. Chiarello D.I., Abad C., Rojas D., Toledo F. et al. Oxidative stress: normal pregnancy versus preeclampsia. Biochim Biophys Acta (BBA) Mol Basis Dis. 2018. doi: https://doi.org/10.1016/J.BBADIS.2018.12.005 .

13. Mitchell M.D., Peiris H.N., Kobayashi M., Koh Y.Q., et al. Placental exosomes in normal and complicated pregnancy. Am J Obstet Gynecol. 2015; 213 (4): S173-S81. doi: https://doi.org/10.1016/j.ajog.2015.07.001 .

14. Skripnichenko Yu.P., Baranov 1.1., Vysokikh M.Yu. Determination of the blood level of mitochondrial DNA for the prediction of pregnancy complication. Akusherstvo i Ginekologiya [Obstetrics and Gynecology]. 2018; (2): 44-9. (in Russian)

15. Vishnyakova P.A., Sukhanova Yu.A., Mikaelyan A.G., Bulatova Yu.S., Pyataeva S.V., Balashov I.S., Borovikov P.I., Tetruashvili N.K., Vyssokikh M.Yu. Fetal growth restriction and markers for mitochondrial dysfunction. Akusherstvo i Ginekologiya [Obstetrics and Gynecology]. 2018; (6): 31-6. doi: https://dx.doi.org/10.18565/aig.2018.6.31-36 (in Russian)

16. Desrochers L.M., Bordeleau F., Reinhart-King C.A., Cerione R.A., et al. Microvesicles provide a mechanism for intercellular communication by embryonic stem cells during embryo implantation. Nat Commun. 2016; 7. doi: https://doi.org/10.1038/ncomms11958 .

17. Mincheva-Nilsson L., Baranov V. Placenta-derived exosomes and syncytiotrophoblast microparticles and their role in human reproduction: immune modulation for pregnancy success. Am J Reprod Immunol. 2014; 72: 440-57. doi: https://doi.org/10.1111/aji.12311 .

18. Goswamia D., Tannetta D.S., Magee L.A., Fuchisawa A., et al. Excess syncytiotrophoblast microparticle shedding is a feature of early-onset pre-eclampsia, but not normotensive intrauterine growth restriction. Placenta. 2006; 27 (1): 56-61. doi: https://doi.org/10.1016/j.pla-centa.2004.11.007 .

All articles in our journal are distributed under the Creative Commons Attribution 4.0 International License (CC BY 4.0 license)