The effectiveness of different methods in the detection of high-grade squamous intraepithelial lesions of cervix uteri
AbstractObjective. To determine the frequency of high-risk human papillomavirus (hr-HPV) among women of Kemerovo region and assess the effectiveness of different methods in the detection of high-grade squamous intraepithelial cervical lesions (HSIL).
Material and methods. The cross-sectional study covered 300 women aged 25 to 59 years (mean age 37.01+8.97 years). Every woman carried out cytology and liquid-based cytology, detection of high-risk HPV DNA (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 genotypes) by PCR real time after physician-collected and self-collected vaginal discharge using Qvintip device, and colposcopy. Women with abnormal results were offered to carrying out target biopsy for histological verification of the diagnosis.
Results. The incidence of hr-HPV was 31.3% among women of Kemerovo region, in half of the cases there was a combination of several HPV genotypes (55.4%). The most common were HPV16 (35.1%), HPV52 (25.5%) and HPV31 (23.4%). Presence of HPV 16 was significantly more prevalent in pre-cancerous lesions (p=0.025). The most sensitive method in detecting HSIL was the detection of hr-HPV DNA in physician-collected samples (91.3%), similar result in case of self-collected material using Qvintip device (87.0%), sensitivity of the cytological method - 43.5%.
Conclusion. Women in Kemerovo region have a high prevalence of HPV-infection with a prevalent of 16, 52 and 31 genotypes. Detection of hr-HPV DNA is the most sensitive technique in detecting HSIL. The result of HPV testing after self-collected samples using Qvintip is comparable to the traditional method (physician-collected samples) and can be used as an alternative method in screening of cervical cancer.
Keywords:squamous intraepithelial lesion of the cervix uteri, human papillomavirus, cervical cancer
DOI: 10.24411/2303-9698-2017-00008