Preimplantation genetic screening in patients with recurrent abortion: risk factors for embryo aneuploidy

• Korotchenko O.E.
• Syrkasheva A.G.
• Kalinina E.A.

Abstract

The aim of the study was to investigate factors that affect for embryo aneupLoidy frequency in patients with recurrent miscarriage and infertility.

Material and methods. 96 patients with recurrent miscarriage (2 or more miscarriages in anamnesis) and infertility were included in the prospective cohort study. ALL of them were treated for infertility by in vitro fertilization method (IVF) and underwent preimplantation genetic screening (PGS) by genome-wide hybridization method with blastocyst trophoectoderm ceLLs study.

Results. PGS was performed for 315 embryos, of which 155 (49%) had different aneupLoidies. In embryo aneupLoidy group patients median age was higher (35.6+6.5 years compared with 33.6+5.1 years, p=0.0011), and average number of mature oocytes was Lower (8.4+4.6 compared with 9.7+4.4, p=0.0094). ThreshoLd age for embryo aneupLoidy risk was 38 years. Proportion of cycLes with cancelation of transfer due to absence of eupLoid embryos was significantLy higher in patients oLder than 38 years: 19 of 39 cycLes (48.7%) in patients of Late reproductive age and 9 of 57 (15.8%) in group of patients of earLy reproductive age (p=0.0004). As a resuLt of PGS realization in patients of different ages, frequency of cLinicaL pregnancy (60% and 62.5%) and Live birth (40% and 50%) were comparabLe.

Conclusion. Main factors affecting aneupLoidy frequency in patients with recurrent miscarriage and infertiLity are age and number of obtained oocytes. In patients of Late reproductive age (38 years and oLder), percentage of aneupLoid bLastocysts and proportion of cycLes with canceLLation of embryo transfer in the absence of eupLoid embryos are significantLy higher. Subject to avaiLabiLity at Least 1 eupLoid embryo, assisted reproductive technologies (ART) programs outcomes are comparabLe in patients of different ages.

Keywords:infertility, recurrent miscarriage, assisted reproductive technologies, preimplantation genetic screening, array comparative genomic hybridization

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